Abstract
The use of wet and dry ashing procedures to decompose marine biological tissues and to degrade organoselenium compounds to inorganic selenium for analysis by fluorescence spectroscopy were investigated. Wet ashing with nitric and perchloric (5:2 v v ) or nitric-sulfuric-perchloric (5:2:0.5 v v ) acid mixtures released the largest percentage of selenium from fish tissue and quantitatively degraded organoselenium compounds to inorganic selenium. The selenium concentrations found when standard reference materials were ashed with these mixtures were in agreement with the certified values.
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