Decomposition of leaf litter mixtures in streams: effects of component litter species and current velocity

Abstract

The effects of mixing different leaf litter species on litter decomposition in streams have received considerable attention in recent years. However, contrasting results have been reported and the mechanisms behind the effects of litter diversity have been poorly examined. We compared the decomposition rates and associated fungi for two contrasting litter species, when incubated individually and in mixture, at two different current velocities. Coarse-mesh bags with alder litter individually, oak litter individually and with a mixture of both were incubated in a forest headwater stream over 32 days, under fast or slow current velocities. We determined litter decomposition rates, microbial oxygen consumption rates, and aquatic hyphomycete sporulation rates, species richness and community composition; litter species in the mixture were processed individually. Our results provided weak evidences for diversity effects on leaf litter decomposition. Generally, litter decomposition was unaffected by mixing contrasting litter species, with litter species in the mixture decomposing at the same rate as when incubated individually at both current velocities. The same pattern was observed for microbial variables. Decomposition rates and microbial colonization and activity depended primarily on the traits of the target litter species and were not affected by those of the companion species. However, litter-mixing effects were detected on oak litter at late decomposition stages under fast current velocity conditions, suggesting that both current velocity and the incubation time might influence diversity effects on litter decomposition in streams. This finding contributes to explain the lack of litter-mixing effects reported previously by many studies.