Abstract
ABSTRACTBackground: The aim of our investigation was to estimate the stability of α-tocopheryl O-glycosides in relation to activity of exoglycosidases in selected rat tissues. Material and Methods: Acetylated glycosides were obtained in glucosidation of α-tocopherol using the Helferich method. The activity of exoglycosidases was determined by the Zwierz et al. method. Protein concentrations were determined by the biuret method. The concentration of released α-tocopherol was determined with the HPLC method. Results: The comparison of the amount of released α-tocopherol with the amount of released p-nitrophenol shows that glycoside bound in 2a–5a derivatives of α-tocopherol undergoes hydrolysis significantly harder than in appropriate 2b–5b p-nitrophenyl derivatives. Conclusion: The results indicate that tocopheryl O-glycosides are more resistant to enzymatic hydrolysis than appropriate p-nitrophenol O-glycosides 2a–5a. Among examined tocopheryl O-glycosides, galactoside 4 is the only compound that caused the significant increase in tocopherol concentration, as compared to its endogenic content.
Highlights
Vitamin E (α-tocopherol 1) (Fig. 1) is regarded as the most important low-molecular lipophilic antioxidant that protects cellular membranes and other phospholipid structures against the destructive activity of oxygen-centered free radicals (Azzi and Stocker 2000; Wang and Quinn 1999)
The present paper shows further data concerning the persistence of α-tocopheryl O-glycosides (2a–5a) (Fig. 2) in the presence of exoglycosidases present in chosen rat tissues
Determination was conducted using a large excess of p-nitrophenyl glycosides and the amount of released p-nitrophenyl is proportional to the activity of the appropriate enzyme
Summary
Vitamin E (α-tocopherol 1) (Fig. 1) is regarded as the most important low-molecular lipophilic antioxidant that protects cellular membranes and other phospholipid structures against the destructive activity of oxygen-centered free radicals (Azzi and Stocker 2000; Wang and Quinn 1999). The investigations carried out for decades showed that administration of vitamin E helps in the treatment of such diseases as atherosclerosis and circulatory disorders. It inhibits prostate proliferation and delays progress of Alzheimer and Parkinson diseases and many other disorders connected with disturbances of free radical levels in the organism (Hasty et al 2007; Negri et al 1991; Vatassery et al 1999). The concentration of released α-tocopherol was determined with the HPLC method. Among examined tocopheryl O-glycosides, galactoside 4 is the only compound that caused the significant increase in tocopherol concentration, as compared to its endogenic content
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