Decolourization of Textile Azo Dyes by a Newly Isolated Bacterial Strain

Abstract

Textile industries make use of large quantities of azo dyes for various ranges of processing, which are recalcitrant in nature and resistant to degradation. The present study examined on screening of adaptive bacterial species from textile effluent polluted soil and its efficacy to decolourize commonly used textile azo dyes. Azo dye degrading adaptive species have been selected by the use of nutrient agar medium supplemented with azo dye with 100 ppm. A Bacterial isolate SKB16 was chosen and experimented to decolourize Reactive Yellow and Reactive Red F3B dyes. The optimization of decolorization conditions which includes pH, temperature and dye concentration were studied. The adaptive species SKB16 showed maximum decolourization of Reactive yellow and Reactive red F3B in 100 ppm concentration at pH 7, temperature 37°C at 98 hrs of incubation. Biotransformation of the azo dyes were assessed by characterizing the metabolites formed after degradation through Fourier transform infrared spectroscopy (FT-IR) and High-performance liquid chromatography (HPLC). FT-IR and HPLC analysis of dye degraded metabolites by the bacterial isolate SKB16 proved that the decolourization process was due to degradation .This study illustrates that potential of the newly isolate adaptive strain of Enterobacter sps SKB16 to be employed for the treatment of textile dye containing effluents.