Decolorization of azo dyes by a salt-tolerant Staphylococcus cohnii strain isolated from textile wastewater

Abstract

The salt-tolerant Staphylococcus cohnii strain, isolated from textile wastewater, has been found effective on decolorizing several kinds of azo dyes with different structures. The optimal conditions for azo dye acid red B (ARB) decolorization by S. cohnii were determined to be pH = 7.0 and 30°C. The decolorization efficiency increased with the increase of the salinity concentration, and around 90% of ARB (100 mg·L−1) could be decolorized in 24 h when the salinity concentration was up to 50 g·L−1. Moreover, the strain could still decolorize 19% of ARB in 24 h even when the NaCl concentration was increased to 150 g·L−1. Meanwhile, the dependence of the specific decolorization rate by S. cohnii on the ARB concentration could be described with Michaelis-Menten kinetics (Km = 585.7mg·L−1, Vmax = 109.8 mg·g cell−1·h−1). The addition of quinone redox mediator, named 2-hydroxy-1,4-naphthoquinone and anthraquinone-2,6-disulfonate, significantly accelerated the decolorization performance of S. cohnii. Furtherly, the activities of azoreductase (0.55 μmol·mg protein−1·min−1) and Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase (8.9 μmol·mg protein−1·min−1) have been observed in the crude cell extracts of S. cohnii. The decolorization products of ARB were analyzed by HPLC-MS, and the results indicated the reductive pathway was responsible for azo dye decolorization by S. cohnii.