Deciphering bioprocess responses in organic phosphorus mineralization to different antibiotic stresses: Interaction mechanisms mediated by microbial succession and extracellular polymeric substances and regulatory patterns

Abstract

Understanding the impacts and mechanisms of different antibiotics on organic phosphorus (OP) mineralization is crucial for promoting the rational utilization of resources and protecting the ecological environment. In this study, the effects of four commonly used antibiotics (sulfadiazine, ciprofloxacin, erythromycin, and ampicillin) on the mineralization of OP were explored using16S rRNA gene sequencing technology. The results showed that ciprofloxacin, erythromycin, and ampicillin negatively affected the mineralization capacity of OP, whereas sulfadiazine positively influenced OP mineralization. The content and composition of extracellular polymeric substances (EPS) and microbial phenotypes (biofilm formation and stress tolerance) were directly correlated with differences in OP mineralization capacity. Microbial diversity, network complexity and stability, and key microorganisms indirectly influenced OP mineralization by regulating EPS content and composition and microbial phenotypes. In summary, this study reveals specific impacts of different antibiotics on OP mineralization, offering valuable insights for addressing “phosphorus limitation” and “phosphorus load” amid various antibiotic stresses.