Abstract
The objective of this research was to examine the effectiveness of coconut water with various egg yolk concentrations in maintaining the viability of epididymal spermatozoa of crossbreed cattle preserved at 5oC. Five testis with epididymides of crossbreed cattle were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure methods of cauda epididymides with saline solution (0.9% NaCl). Collected-spermatozoa was equally divided in volume into four tubes and diluted with lactose extender containing 20% egg yolk (control), 90% coconut water + 10% egg yolk (CWEY10), 85% coconut water + 15% egg yolk (CWEY15), and 80% coconut water + 20% egg yolk (CWEY20), repectively. Diluted-spermatozoa was stored in refrigerator at 5oC. Quality of dilutedspermatozoa including percentages of motile spermatozoa (MS), live spermatozoa (LS), spermatozoa with intact plasma membrane (IPM) were evaluated every day during four days of storage. Data were analyzed by using completely randomized design with four treatments and five replicates. Means were compared with significant difference test at 0.05 significant level. Results of this study showed that mean of spermatozoa concentration, percentage of MS, percentage of LS, percentage of spermatozoa abnormal, and percentage of IPM of crossbreed cattle fresh epididymal spermatozoa were 1,414 million cell/ml, 72%, 85%, 9%, and 90%, respectively. At day-4 of the storage, percentages of MS, LS, and IPM of control (43, 52.2, 59.2%) and CWEY20 (42, 52, 59%) were significantly (P<0.05) higher than CWEY10 (33, 45.4, 52.8%) and CWEY15 (37, 50, 54.6%). In conclusion, lactose and CWEY20 extenders could be maintaining the quality of epidydimal spermatozoa of crossbreed cattle for three days preservation at 5oC and two days for CWEY10 and CWEY15.
Highlights
Jurnal Veteriner compared with significant difference test at 0.05 significant level
The objective of this research was to examine the effectiveness of coconut water with various egg yolk concentrations in maintaining the viability of epididymal spermatozoa of crossbreed cattle preserved at 5oC
Results of this study showed that mean of spermatozoa concentration, percentage of motile spermatozoa (MS), percentage of live spermatozoa (LS), percentage of spermatozoa abnormal, and percentage of intact plasma membrane (IPM) of crossbreed cattle fresh epididymal spermatozoa were 1,414 million cell/ml, 72%, 85%, 9%, and 90%, respectively
Summary
Reproduksi dan Pemuliaan Ternak, Jurusan Peternakan, Fakultas Pertanian, Universitas Lambung Mangkurat, Banjarbaru. Pengencer dasar laktosa terdiri atas: 9 g laktosa dan 1,37 g fruktosa yang dilarutkan dengan akuabidestilata hingga mencapai volume 100 mL. Pengencer laktosa lengkap terdiri atas: 80% pengencer dasar laktosa + 20% kuning telur ayam ras + antibiotik (1.000 μg penisilin dan 1.000 IU streptomisin setiap mililiter pengencer). Pengencer air kelapa muda terdiri atas: air kelapa muda + kuning telur ayam ras + antibiotik (1.000 μg penisilin dan 1.000 IU streptomisin setiap mililiter pengencer). Persentase air kelapa muda dan kuning telur ayam ras sesuai dengan perlakuan. Spermatozoa hasil koleksi dibagi ke dalam empat buah tabung reaksi dengan volume yang sama, kemudian diencerkan sesuai dengan perlakuan. Seluruh tabung reaksi yang berisi spermatozoa yang telah diencerkan sesuai dengan perlakuan ditutup rapat kemudian dimasukkan ke gelas piala yang berisi air bersih dan disimpan di dalam refrigerator lemari es pada suhu sekitar 5oC. Contoh masing-masing perlakuan dievaluasi kualitasnya setiap hari hingga persentase spermatozoa motil 40% (berdasarkan SNI 4869.1:2008)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
