DAYA ANTIBAKTERI EKSTRAK ETANOL DAUN SENGGANI (Melastoma affine D. Don)

Ika Trisharyanti Dian Kusumowati,Rosita Melannisa,Angga Prasetyawan

doi:10.23917/biomedika.v6i2.278

Abstract

Melastoma affine D. Don had some activities such as anthelmintic, antibacteria, antiinfiammation, antifungal, and antitumor. The aims of this research was determine antibacteria activity of ethanolic extract of Melastoma affine D. Don. The antimicrobial activity was tested by solid dilution method to get Minimum Inhibition Concentration (MIC). The compounds in Melastoma affine D. Don was analyzed by tube test method and Thin Layer Chromatography (TLC) with chloroform : methanol : formic acid (8,5:1,5:0,5) as mobile phase and silica gel GF254 as stationary phase. The result showed ethanolic extract of Melastoma affine D. Don contains alkaloid, polyphenol, fiavonoid, saponin, and essential oil. The MIC of Senggani against Staphylococcus aureus was 2% and 3% against Escherichia coli and the extract could not inhibit Staphylococcus aureus and Escherichia coli multiresistant until concentration 7% extract ethanol.Keywords: Melastoma affine D. Don, Staphylococcus aureus, Escherichia coli

Highlights

PENDAHULUAN Penyakit infeksi merupakan ancaman bagi kelangsungan hidup manusia
The antimicrobial activity was tested by solid dilution method to get Minimum Inhibition Concentration
The compounds in Melastoma af ne D. Don was analyzed by tube test method

Summary

Introduction

PENDAHULUAN Penyakit infeksi merupakan ancaman bagi kelangsungan hidup manusia. Beberapa bakteri yang dapat menyebabkan penyakit diantaranya adalah Staphylococcus aureus (S. aureus) dan Escherichia coli (E. coli) (Jawetz et al, 2005). Hasil skrining tokimia menunjukkan bahwa ekstrak metanol daun Senggani mengandung senyawa tanin, avonoid, steroid, saponin, dan kuinon. Fraksi etil asetat ekstrak metanol daun Senggani mempunyai aktivitas antibakteri terhadap bakteri S. aureus, E. coli, dan Bacilus cereus dengan KHM (Kadar Hambat Minimal) untuk S. aureus 0,78-1,56%, untuk E. coli 25-50%, dan Bacilus cereus 0,390,78% dengan metode difusi agar (Titi et al, 2007).

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Conclusion