Abstract
Minerals and trace elements play vital role in the biological functions for all organisms including human and other mammals. Therefore, imbalance in the mineral and/or trace element levels may cause formation of several diseases including cancer, diabetes, cardiovascular diseases, and neurological disorders. ICP-MS (inductively coupled plasma – mass spectrometry) is described as the most sensitive and accurate method. Here we reported an effective and fast protocol as method validation to evaluate trace element and minerals via ICP-MS in the mammalian tissue and serum samples. Our data showed that minimum relative standard deviation (RSD) values with the ICP-MS were observed when we used microwave digestion with the SUPRAPUR® grade nitric acid at the lower dilution rates. Our protocol validation may help researchers to measure trace elements and minerals in the mammalian samples fast, easily and accurately. EMSURE® grade HNO3 caused cross contamination in the serum and tissue samples. Our protocol validation may help researchers to measure trace elements and minerals in the mammalian samples fast, easily and accurately.
Highlights
Dataset of the analyzing trace elements and minerals via inductively coupled plasma mass spectrometry (ICP-MS): Method validation for the mammalian tissue and serum samples
Our data showed that minimum relative standard deviation (RSD) values with the ICP-MS were observed when we used microwave digestion with the SUPRAPUR® grade nitric acid at the lower dilution rates
Trace elements and minerals were measured via ICP-MS
Summary
Data format Description of data collection Data source location Data accessibility. Biology ICP-MS, minerals and trace elements, method validation Table Graph Figure ICP-MS Microwave digestion Animal experiments Statistical analysis Raw and Analyzed Samples were digested via microwave digestion. Value of the Data This data compares different methods and chemicals to find most accurate technique to measure minerals and trace elements in the mammalian samples. The data are potentially useful for researchers who need to prepare mammalian tissue and serum samples via acidic digestion for ICP-MS. These data gave a detailed and complete set of experiments that could be used to measure Ag, Al, As, Ba, Be, Ca, Cd, Co, Cr, Cs, Cu, Fe, Ga, K, Li, Mg, Mn, Na, Ni, Pb, Rb, Se, Sr, Tl, U, V, and Zn concentrations in all types of biological samples via microwave digestion and ICP-MS. In this report we firstly compare efficacy and accuracy of alkali dilution and acidic digestion methods in mammalian samples including brain, liver, testis, lung, kidney, heart, spleen and serum, alkali digestion was not able to digest tissue samples (supplementary data). The best dilution rates are 1/ 10 to 1/20 to obtain the most accurate data according to the relative standard deviation values (Figs. 1e8, Tables 1e8)
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