Dataset of protein changes induced by cold acclimation in red clover (Trifolium pratense L.) populations recurrently selected for improved freezing tolerance

Marie Bipfubusa,Solen Rocher,Annick Bertrand,Yves Castonguay,Jenny Renaut

doi:10.1016/j.dib.2016.06.003

Marie Bipfubusa, Solen Rocher + Show 3 more

Open Access

https://doi.org/10.1016/j.dib.2016.06.003

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Abstract

The data provide an overview of proteomic changes in red clover (Trifolium pratense L.) in response to cold acclimation and recurrent selection for superior freezing tolerance. Proteins were extracted from crowns of two red clover cultivars grown under non-acclimated or cold-acclimated conditions, and plants obtained from the initial genetic background (TF0) and from populations obtained after three (TF3) and four cycles (TF4) of recurrent selection for superior freezing tolerance. Proteins were analyzed using a two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled to mass spectroscopy (MS and MS/MS). Differentially regulated proteins were subsequently identified using MALDI TOF/TOF analysis. The data are related to a recently published research article describing proteome composition changes associated with freezing tolerance in red clover, “A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)” (Bertrand et al., 2016 [1]). They are available in the ProteomeXchange Consortium database via the PRIDE partner repository under the dataset identifier PRIDE: PXD003689.

Highlights

Dataset of protein changes induced by cold acclimation in red clover (Trifolium pratense L.) populations recurrently selected for improved freezing tolerance
The data provide an overview of proteomic changes in red clover (Trifolium pratense L.) in response to cold acclimation and recurrent selection for superior freezing tolerance
Proteins were extracted from crowns of two red clover cultivars grown under nonacclimated or cold-acclimated conditions, and plants obtained from the initial genetic background (TF0) and from populations obtained after three (TF3) and four cycles (TF4) of recurrent selection for superior freezing tolerance

Summary

Data accessibility

Proteins were separated using a two Dimensional fluorescence Difference Gel Electrophoresis (2D-DIGE) and analyzed by mass spectroscopy (MS and MS/MS). Proteins were separated using 2D-DIGE and analyzed using MS, and the differentially regulated proteins (regarding selection for superior freezing tolerance and cold acclimation status) were identified using MALDI TOF/TOF analysis. The data are useful to pursue further proteomic studies on the development of freezing tolerance in plants and to understand the cold acclimation process. Our proteomic data may be combined with genomic and transcriptomic data to provide better insights into the improved cold acclimation ability of recurrently selected red clovers for superior freezing tolerance. The data provide an overview of changes in crown proteome that are associated with the improvement of freezing tolerance in red clover (Trifolium pratense L.) through cold acclimation and recurrent selection for superior freezing tolerance [1].

Plant recurrent selection and cold acclimation

Protein extraction and quantification

Statistical analyses