A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.).

Annick Bertrand,Yousef Papadopoulos,Solen Rocher,Jenny Renaut,Marie Bipfubusa,Aleksandra Szopinska-Morawska,Yves Castonguay

doi:10.1186/s12870-016-0751-2

Annick Bertrand, Yousef Papadopoulos + Show 5 more

Open Access

https://doi.org/10.1186/s12870-016-0751-2

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Abstract

BackgroundImprovement of freezing tolerance of red clover (Trifolium pratense L.) would increase its persistence under cold climate. In this study, we assessed the freezing tolerance and compared the proteome composition of non-acclimated and cold-acclimated plants of two initial cultivars of red clover: Endure (E-TF0) and Christie (C-TF0) and of populations issued from these cultivars after three (TF3) and four (TF4) cycles of phenotypic recurrent selection for superior freezing tolerance. Through this approach, we wanted to identify proteins that are associated with the improvement of freezing tolerance in red clover.ResultsFreezing tolerance expressed as the lethal temperature for 50 % of the plants (LT50) increased markedly from approximately −2 to −16 °C following cold acclimation. Recurrent selection allowed a significant 2 to 3 °C increase of the LT50 after four cycles of recurrent selection. Two-dimensional difference gel electrophoresis (2D-DIGE) was used to study variations in protein abundance. Principal component analysis based on 2D-DIGE revealed that the largest variability in the protein data set was attributable to the cold acclimation treatment and that the two genetic backgrounds had differential protein composition in the acclimated state only. Vegetative storage proteins (VSP), which are essential nitrogen reserves for plant regrowth, and dehydrins were among the most striking changes in proteome composition of cold acclimated crowns of red clovers. A subset of proteins varied in abundance in response to selection including a dehydrin that increased in abundance in TF3 and TF4 populations as compared to TF0 in the Endure background.ConclusionRecurrent selection performed indoor is an effective approach to improve the freezing tolerance of red clover. Significant improvement of freezing tolerance by recurrent selection was associated with differential accumulation of a small number of cold-regulated proteins that may play an important role in the determination of the level of freezing tolerance.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0751-2) contains supplementary material, which is available to authorized users.

Highlights

Improvement of freezing tolerance of red clover (Trifolium pratense L.) would increase its persistence under cold climate
Recurrent selection improves freezing tolerance of red clover Our results clearly demonstrated the capacity for substantial cold acclimation in red clover with a LT50 ranging from an average of −3 °C in non-acclimated plants down to −14 °C in plants overwintering under natural conditions in January
They revealed that recurrent selection performed indoor is an effective approach to improve the freezing tolerance of red clover since the LT50 was improved by up to 3 °C after four cycles of selection (TF4) in the two genetic backgrounds

Summary

Introduction

Improvement of freezing tolerance of red clover (Trifolium pratense L.) would increase its persistence under cold climate. We assessed the freezing tolerance and compared the proteome composition of non-acclimated and cold-acclimated plants of two initial cultivars of red clover: Endure (E-TF0) and Christie (C-TF0) and of populations issued from these cultivars after three (TF3) and four (TF4) cycles of phenotypic recurrent selection for superior freezing tolerance. Through this approach, we wanted to identify proteins that are associated with the improvement of freezing tolerance in red clover.

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