Abstract
IR spectroscopic images were recorded using an Equinox Bruker FTIR spectrometer coupled to a Hyperion 3000 imaging system equipped with a mercury cadmium telluride (MCT)-based focal plane array (FPA) detector of 64×64 pixels (Bruker Optik, Ettlingen, Germany). Images containing 4096 IR spectra at 8 cm-1 spectral resolution were acquired by coadding 256 interferograms in about 5 minutes. All the data processing was carried out by the program “Kinetics” running under MatLab. The “Kinetics” software, previously used for FTIR spectrum processing and analysis, was extended for the processing of FPA-acquired FTIR data. Different types of images can be generated, either based on the absorbance at 1 wavenumber or the ratio of the absorbances at 2 wavenumbers or more advanced combination of the data. We show here that protein secondary structure content evaluated as described in [1] sheds light on the molecular determinants that allow the differentiation between sub-structures in tissues. Similarly we shall illustrate heterogeneity in prostate cancer cells (PC-3) in culture based on secondary structure imaging.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
