Abstract
This data article presents the differences observed between the myristoylated and non-myristoylated forms of the neuronal calcium sensor protein, neurocalcin delta (NCALD). Analysis of the myristoylated and non-myristoylated versions of the protein by mass spectrometry provided difference in mass values consistent with addition of myristoyl group. In the presence of calcium, mobility retardation was observed upon electrophoresis of the protein in native gels. The retardation was dose-dependent and was exhibited by both the myristoylated and non-myristoylated forms of the protein.
Highlights
This data article presents the differences observed between the myristoylated and non-myristoylated forms of the neuronal calcium sensor protein, neurocalcin delta (NCALD)
In the presence of calcium, mobility retardation was observed upon electrophoresis of the protein in native gels
Mass spectroscopy: Bruker Microflex LRF MALDI-TOF Electrophoresis: Bio-Rad miniPROTEAN Analyzed For mass spectrometry, the samples were co-crystallized in 1:1 mixture of sinapinic acid and matrix solution containing acetonitrile, water and trifluoroacetic acid
Summary
Jeffrey Viviano a, Anuradha Krishnan a, Hao Wu a, Venkat Venkataraman a,b,n a Graduate School of Biomedical Sciences, Rowan University, Stratford, NJ 08084, USA b School of Osteopathic Medicine, Rowan University, Stratford, NJ 08084, USA article info. Article history: Received 19 January 2016 Received in revised form 21 February 2016 Accepted 3 March 2016 Available online 11 March 2016 abstract. This data article presents the differences observed between the myristoylated and non-myristoylated forms of the neuronal calcium sensor protein, neurocalcin delta (NCALD). In the presence of calcium, mobility retardation was observed upon electrophoresis of the protein in native gels. The retardation was dose-dependent and was exhibited by both the myristoylated and non-myristoylated forms of the protein.
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