Abstract
Endurance exercise is securely linked to muscle metabolic adaptations including enhanced mitochondrial function (“Effects of exercise on mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle” [1], “Effects of exercise on mitochondrial content and function in aging human skeletal muscle” [2]). However, the link between exercise intensity and mitochondrial function in aging muscle has not been fully investigated. In order to understand how strenuous exercise affects mitochondrial function in aged mice, male C57BL/6 mice at age 24 months were randomly assigned to 3 groups: non-exercise (NE), low-intensity (LE) and high-intensity treadmill exercise group (HE). Mitochondrial complex activity and respiration were measured to evaluate mitochondrial function in mouse skeletal muscle. The data described here are related to the research article entitled “Strenuous exercise induces mitochondrial damage in skeletal muscle of old mice” [3].
Highlights
Data on mitochondrial function in skeletal muscle of old mice in response to different exercise intensity
In order to understand how strenuous exercise affects mitochondrial function in aged mice, male C57BL/6 mice at age 24 months were randomly assigned to 3 groups: non-exercise (NE), low-intensity (LE) and high-intensity treadmill exercise group (HE)
The data described here are related to the research article entitled “Strenuous exercise induces mitochondrial damage in skeletal muscle of old mice” [3]. & 2016 The Authors
Summary
Each mice was sacrificed and soleus skeletal muscle tissues were quickly dissected. 5 mL ice-cold 0.25 M sucrose, 1 mM EDTA, 5 mM HEPES, 0.2% bovine serum albumin (BSA), 13 units/10 mL collagenase (pH 7.4) isolation media 1 (IM1) was added and the muscle was minced with scissors. The supernatant was saved on ice and the pellet was resuspended in a 1:10 ratio (w/v) of IM1 and spun again under the same conditions (4 °C for 10 min at 700g). The pellet was resuspended using a smooth-headed Potter–Elvehjem pestle in 15 mL of 0.25 M sucrose and 1 mM EGTA (pH 7.4) isolation media 2 (IM2). The mixture was again spun under the same conditions (4 °C for 10 min at 12,000g), the supernatant discarded, and fat removed. The pellet was resuspended in 200 μL of 0.25 M sucrose and 2 mM EDTA, pH 7.4 isolation media 3 (IM3) buffer using a smooth-headed Potter–Elvehjem pestle.
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