Abstract
The present dataset describes a detailed protocol to isolate mesenchymal cells from human fat without the use of collagenase. Human fat specimen, surgically cleaned from non-fat tissues (e.g., blood vessels) and reduced into smaller fat pieces of around 1–3 mm size, is incubated in complete culture media for five to seven days. Then, cells started to spread out from the fat explants and to grow in cultures according to an exponential pattern. Our data showed that primary mesenchymal cells presenting heterogeneous morphology start to acquire more homogenous fibroblastic-like shape when cultured for longer duration or when subcultured into new flasks. Cell isolation efficiency as well as cell doubling time were also calculated throughout the culturing experimentations and illustrated in a separate figure thereafter. This paper contains data previously considered as an alternative protocol to isolate adipose-derived mesenchymal stem cell published in “Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent” [1].
Highlights
The present dataset describes a detailed protocol to isolate mesenchymal cells from human fat without the use of collagenase
Our data showed that primary mesenchymal cells presenting heterogeneous morphology start to acquire more homogenous fibroblastic-like shape when cultured for longer duration or when subcultured into new flasks
Cell isolation efficiency as well as cell doubling time were calculated throughout the culturing experimentations and illustrated in a separate figure thereafter
Summary
The below data provide a detailed and reproducible collagenase-free protocol to isolate mesenchymal stromal cells from human adipose tissue. These data enable researchers to isolate various cell types populating fat. These data offer the possibility to isolate specific primary cell cultures with a reduced and efficient cell isolation yield. The data presented in this paper correspond to the isolation of mesenchymal stromal cells without digesting human fat pieces with collagenase. These data were considered as an alternative method to isolate stem cells from human adipose tissues in our previously published manuscript [1] These data were considered as an alternative method to isolate stem cells from human adipose tissues in our previously published manuscript [1] (Figs. 1–3)
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