Abstract
Valproic acid/sodium valproate (VPA), a drug primarily used for treatment of seizure disorders, is recognized as an efficient epigenetic agent, inducing inhibition of histone deacetylases, promoting changes in the methylation status of DNA and histones, and affecting chromatin structure. In addition to these epigenetic effects, molecular affinity of VPA for histone H1 has been proposed based on thermal denaturation, fluorescence spectroscopy and circular dichroism assays. VPA interactions with DNA and histones using Fourier transform infrared (FTIR) microspectroscopy and high-performance polarization microscopy that are not related to the effects promoted on epigenetic markers have been recently explored. Data in this article provide supplementary information for a better understanding of the resulting FTIR spectra for mixtures of VPA and histones H1 and H3 and of the potential effect of VPA directly on histones that has been reported in the literature.
Highlights
Valproic acid/sodium valproate (VPA), a drug primarily used for treatment of seizure disorders, is recognized as an efficient epigenetic agent, inducing inhibition of histone deacetylases, promoting changes in the methylation status of DNA and histones, and affecting chromatin structure
Ten-μL drops of a solution of 5 mg of histone H1 dissolved in 84 μL of Milli-Q water and of a solution of 2 mg of histone H3 dissolved in 69 μL of Milli-Q water were treated to the VPA preparations
The same process was applied to VPA-histone H1 and VPA-histone H3 mixtures (5-μL each)
Summary
Valproic acid/sodium valproate (VPA), a drug primarily used for treatment of seizure disorders, is recognized as an efficient epigenetic agent, inducing inhibition of histone deacetylases, promoting changes in the methylation status of DNA and histones, and affecting chromatin structure. These data provide indication of VPA interaction with histone molecules;
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