Abstract
This data article is associated with the research article “Evaluating the impact of methionine-enriched diets in the liver of European seabass through label-free shotgun proteomics”. Here it is described the data obtained from proteomic analysis of 36 European seabass juveniles (3 fish x 3 replicate tanks) after 18 days of feeding with experimental diets containing four inclusion levels of methionine (Met): 0.77%, 1%, 1.36% and 1.66% Met (w/w). We analysed this dataset and compared it with that obtained during the long-term feeding period i.e., 85 days. Fish liver proteins were digested with trypsin and purified peptides were analysed by LC-MS/MS. Proteins were identified with at least two peptides at 0.1% Decoy false discovery rate (FDR). In this dataset, we present the analysis of the differential abundant proteins (DAP) with significant differences across treatments after 18 days of feeding (One-Way ANOVA, p < 0.05). Treatment's comparisons were also performed between the 18- and 85-days feeding trials through Two-Way ANOVA (p < 0.05). MS/MS raw data are available via ProteomeXChange with identifiers PXD019610 and 10.6019/PXD019610 (18-days dataset); and PXD019622 and 10.6019/PXD019622 (85-days dataset). This dataset corresponds to fish sampled after 18-days of experimental trial and is made available to support the study conducted in the afore-mentioned article, by performing the analysis during a short-term period of feeding. The data presented may be further used in other nutritional studies e.g., addressing hepatic changes mediated by Met.
Highlights
This data article is associated with the research article “Evaluating the impact of methionine-enriched diets in the liver of European seabass through label-free shotgun proteomics”
We present the analysis of the differential abundant proteins (DAP) with significant differences across treatments after 18 days of feeding (One-Way ANOVA, p < 0.05)
These label free shotgun proteomics data are important as it represents the first comprehensive dataset on the liver proteome of European seabass in response to dietary methionine, being compared between a short-term and long-term period of feeding i.e., 18 and 85 days of an experimental trial
Summary
Biology Fish proteomics, Label-free quantitative mass spectrometry Figure Table Mass spectrometry, by using reverse phase nano liquid chromatography (Ultimate 3000 system) coupled online to a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Scientific, Bremen, Germany) in data-dependent acquisition mode. Evaluating the impact of methionine-enriched diets in the liver of European seabass through label-free shotgun proteomics. These label free shotgun proteomics data are important as it represents the first comprehensive dataset on the liver proteome of European seabass in response to dietary methionine, being compared between a short-term and long-term period of feeding i.e., 18 and 85 days of an experimental trial. The current proteomics data offer complementary information to classical fish performance indicators, that may be useful in the refinement of amino acid requirements, in this case methionine, assessing fish hepatic proteome changes over a short-term and long-term period of feeding. Relevant information about regulatory mechanisms involved in cell/organism response to methionine, that can be further extended to other teleost fish, besides vertebrate organisms. The current proteomics data offer complementary information to classical fish performance indicators, that may be useful in the refinement of amino acid requirements, in this case methionine, assessing fish hepatic proteome changes over a short-term and long-term period of feeding. As methionine is an indispensable amino acid with an essential role in both fish growth and immune condition/response, these data can help to define future protein indicators/biomarkers of growth performance and/or of immune condition
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