Data in support of global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii.

Micaela Cerletti,Rosana E De Castro,Roberto A Paggi,Ansgar Poetsch,Carina Ramallo Guevara

doi:10.1016/j.dib.2015.04.013

Abstract

This data article provides information in support of the research article “Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii” [1]. The proteome composition of a wt and a LonB protease mutant strain (suboptimal expression) in the archaeon Haloferax volcanii was assessed by a quantitative shotgun proteomic approach. Membrane and cytosol fractions of H. volcanii strains were examined at two different growth stages (exponential and stationary phase). Data is supplied in the present article. This study represents the first proteome examination of a Lon-deficient cell of the Archaea Domain.

Highlights

Article history: Received 20 April 2015 Accepted 20 April 2015 Available online 7 May 2015 abstract. This data article provides information in support of the research article “Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii” [1]
The proteome composition of a wt and a LonB protease mutant strain in the archaeon Haloferax volcanii was assessed by a quantitative shotgun proteomic approach
Membrane and cytosol fractions of H. volcanii strains were examined at two different growth stages

Summary

Strains and culture conditions

HVLON3 is a conditional expression strain which has the tryptophan-regulated promoter (PtnaA) [2] located upstream the lon gene in H. volcanii H26 chromosome (PtnaA-lon-abi). This strain synthesizes very low amounts of Lon and Abi in absence of trp in the culture medium [3]. To obtain the “Lon subproteome”, proteins that changed as a consequence of the Abi mutation were discarded. These strains were grown in minimal medium (Hv-Min) containing uracil (50 μg mlÀ1) [4] in absence of trp at 42 1C 200 rpm. For proteome analysis four independent cultures (biological replicates) of each strain were analyzed and compared

Preparation of cytoplasm and membrane fractions

Findings

In-gel tryptic digestion