Data from Deuterium Magnetic Resonance Imaging Using Deuterated Water-Induced <sup>2</sup>H-Tissue Labeling Allows Monitoring Cancer Treatment at Clinical Field Strength

Abstract

<div>AbstractPurpose:<p>An accurate and noninvasive assessment of tumor response following treatment other than traditional anatomical imaging techniques is essential. Deuterium magnetic resonance spectroscopic (MRS) imaging has been demonstrated as an alternative for cancer metabolic imaging by high-field MRI using deuterium-labeled molecules. The study aim was to use <sup>2</sup>H tissue labeling and deuterium MRI at clinical field strength for tumor visualization and assessment of three anticancer therapies in pancreatic cancer model mice.</p>Experimental Design:<p>MIA PaCa-2 pancreatic carcinoma and C26 colorectal carcinoma models of BALB/c-nu mice was prepared, and repeated deuterium MRI was performed during the first 10 days of free drinking of 30% D<sub>2</sub>O to track <sup>2</sup>H distribution in tissues. <sup>2</sup>H accumulation in the tumor after irradiation, bevacizumab administration, or gemcitabine administration was also measured in MIA PaCa-2–bearing mice. Confirmatory proton MRI, <i>ex vivo</i> metabolic hyperpolarization <sup>13</sup>C-MRS, and histopathology were performed.</p>Results:<p>The mouse's whole-body distribution of <sup>2</sup>H was visible 1 day after drinking, and the signal intensity increased daily. Although the tumor size did not change 1 and 3 days after irradiation, the amount of <sup>2</sup>H decreased significantly. The <sup>2</sup>H image intensity of the tumor also significantly decreased after the administration of bevacizumab or gemcitabine. Metabolic hyperpolarization <sup>13</sup>C-MRS, proton MRI, and <sup>2</sup>H-NMR spectroscopy confirmed the efficacy of the anticancer treatments.</p>Conclusions:<p>Deuterium MRI at 1.5T proved feasible to track <sup>2</sup>H distribution throughout mouse tissues during D<sub>2</sub>O administration and revealed a higher <sup>2</sup>H accumulation in the tumor xenografts. This research demonstrated a promising successful method for preliminary assessment of radiotherapy and chemotherapy of cancer.</p></div>