Abstract
The tumor cell proliferation, migration and invasion were influenced by the interaction between the cancer cells and their microenvironment. In current study, we established two pairs of the primary fibroblast cultures from colorectal adenocarcinoma tissues and the normal counterparts and identified 227 proteins in the colonic fibroblast secretomes; half of these proteins were novel. The mass spectrometry data and analyzed results presented here provide novel insights into the molecular characteristics and modulatory role of colon cancer associated fibroblasts. The data is related to “Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation” by Chen et al. [1].
Highlights
Biology Cancer microenvironment Excel tables Mass spectrometry, data acquired using Synapt G1 mass spectrometer Analyzed Conditioned media were collected, proteins were concentrated using filters The proteins were separated using SDS-PAGE, in-gel tryptic digested and analyzed using LC-MS Shanghai, China The data is available with this article and is related to [1]
Potential exosome protein were revealed by comparing the protein names and accessions with those registered in ExoCarta DB version 4.1 [17] and Vesiclipedia DB version 2.1 [18]
The small interference RNAs and cloning primers are listed in Supplementary Table 5
Summary
We established two pairs of colon cancer-associated fibroblast (CAF) and normal fibroblast (NF) cultures. The peptide mixture from each gel slice was first loaded onto a reverse phase (RP) trap column (C18, 5 μm, 180 μm  20-mm Symmetry C18 nanoAcquity column, Waters) for on-line desalting at a flow rate of 10 μL/min. The proteomic data include the exclusive spectrum count, exclusive unique peptide count, percent coverage, exclusive unique spectrum count and the total spectrum count. Statistics on the spectrum and protein identification reported by Scaffold is summarized in Supplementary Table 4. The spectra and the sequence coverage illustrations were extracted from the Scaffold results
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