Abstract
Here, we present the data from an in-depth analysis of the HSP70 family in the non-model banana during osmotic stress [1]. First, a manual curation of HSP70 sequences from the banana genome was performed and updated on the Musa hub http://banana-genome.cirad.fr/. These curated protein sequences were then introduced into our in-house Mascot database for an in-depth look at the HSP70 protein profiles in banana meristem cultures and roots during osmotic stress. A 2D-DIGE LC MS/MS approach was chosen to identify and quantify the different paralogs and allelic variants in the HSP70 spots.
Highlights
Data for the characterization of the HSP70 family during osmotic stress in banana, a nonmodel crop
We present the data from an in-depth analysis of the HSP70 family in the non-model banana during osmotic stress [1]
Meristem and root proteomes were separated using 2D-DIGE and proteins belonging to the HSP70 features family were identified using LC MS/MS
Summary
Data provides an overview of the identification and quantification of the HSP70 family in a nonmodel plant with special attention to osmotic stress. Manual curation of the banana HSP70 sequences was performed as correct structural annotation of a sequenced genome is essential prior to proteomic analysis. The proteomics approach using 2DE and LC MS/MS was a deciding factor in the successful identification and quantification of the HSP70 paralogs and allelic variants
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