Abstract
In this study, the expression ofArnt, Vtg, CYP4, CYP314 andDhb was analyzed as representative biomarkers after exposingDaphnia magna to a variety of heavy metals,i.e., sodium arsenite, sodium arsenate, and cadmium nitrate. The expression levels of these genes were quantified by semi-quantitative reverse transcription-PCR. In comparison with all chemicals, Cd was showed high toxicity more than other chemicals. Although As(III) and As(V) is similar group, As(III) is high toxicity more than as(V).CYP314 andVtg were specific responsive genes to As(III) and As(V), respectively. Accordingly,CYP314 to As(III) andVtg to As(V) are candidate biomarkers.
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