Abstract
Trillions of microbes representing all kingdoms of life are resident in, and on, humans holding essential roles for the host development and physiology. The last decade over a dozen online tools and servers, accessible via public domain, have been developed for the analysis of bacterial sequences; however, the analysis of fungi is still in its infancy. Here, we present a web server dedicated to the comprehensive analysis of the human mycobiome for (i) translating raw sequencing reads to data tables and high-standard figures, (ii) integrating statistical analysis and machine learning with a manually curated relational database and (iii) comparing the user’s uploaded datasets with publicly available from the Sequence Read Archive. Using 1,266 publicly available Internal transcribed spacers (ITS) samples, we demonstrated the utility of DAnIEL web server on large scale datasets and show the differences in fungal communities between human skin and soil sites.
Highlights
Metagenomics provide a comprehensive view about microbial community structure
We present DAnIEL (Describing, Analyzing and Integrating fungal Ecology to effectively study the systems of Life), the only web server that covers the whole workflow of Internal transcribed spacers (ITS) analysis beginning from raw reads to publication ready figures and tables, contains a relational database for the biological evaluation of statistical findings and allows comparative analysis with public available mycobiome datasets
Whereas the web DAnIEL ITS Analysis Web Server server can be used with ITS samples from all kinds of environments, we started to build the manual curated database with fungal species relevant for humans; many of the species are found in other environmental niches as well
Summary
Metagenomics provide a comprehensive view about microbial community structure. Previous studies have revealed many insights about the diversity, composition, and interaction patterns of bacterial communities. Fungal metagenomics is becoming an essential part for comprehensive human host studies and should be accessible to the whole scientific community without the need of laborious and time-consuming efforts. We present DAnIEL (Describing, Analyzing and Integrating fungal Ecology to effectively study the systems of Life), the only web server that covers the whole workflow of ITS analysis beginning from raw reads to publication ready figures and tables, contains a relational database for the biological evaluation of statistical findings and allows comparative analysis with public available mycobiome datasets. DAnIEL ITS Analysis Web Server server can be used with ITS samples from all kinds of environments, we started to build the manual curated database with fungal species relevant for humans; many of the species are found in other environmental niches as well.
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