Dance Training Improves Cytokine Secretion and Viability of Neutrophils in Diabetic Patients.

Leandro Borges,Maria E P Passos,César M Momesso,Maysa B B Silva,Stuart R Gray,Vinicius C Santos,Tania C Pithon-Curi,Elaine Hatanaka,Renata Gorjão,Kauê C A Lima,Paulo B De Freitas

doi:10.1155/2019/2924818

Abstract

Background Evidence suggests that exercise improves neutrophil function. The decreased functional longevity of neutrophils and their increased clearance from infectious sites contribute to the increased susceptibility to infection and severity of infection observed in patients with diabetes. Objective Herein, we investigated the effects of a dance program on neutrophil number, function, and death in type 2 diabetes mellitus (T2DM) patients and healthy volunteers. Methods Ten patients with T2DM and twelve healthy individuals participated in a moderate-intensity dance training program for 4 months. The plasma levels of leptin, free fatty acids (FFAs), tumour necrosis factor-α (TNF-α), C-reactive protein (CRP), interleukin-1β (IL-1β), and interleukin-1 receptor antagonist (IL-1ra); neutrophil counts; extent of DNA fragmentation; cell membrane integrity; and production of TNF-α, interleukin-8 (IL-8), interleukin-6 (IL-6), and IL-1β in neutrophils were measured before and after training. Results Training reduced plasma levels of TNF-α (1.9-fold in controls and 2.2-fold in patients with T2DM) and CRP (1.4-fold in controls and 3.4-fold in patients with T2DM). IL-1ra levels were higher in the control group (2.2-fold) after training. After training, neutrophil DNA fragmentation was decreased in patients with T2DM (90%), while the number of neutrophils increased (70% in controls and 1.1-fold in patients with T2DM). Conclusion Dance training is a nonpharmacological strategy to reduce inflammation and improve neutrophil clearance in patients with T2DM.

Highlights

Neutrophil apoptosis, an important component of inflammation, regulates neutrophil functional longevity and resolution
Female volunteers with type 2 diabetes mellitus (T2DM) diagnosed according to the criteria established by the American Diabetes Association [8] at least 10 years earlier were recruited from a medical centre specializing in diabetes (Associação Nacional de Assistência ao Diabético (ANAD))
After training, there was an increase in neutrophil tumour necrosis factor-α (TNF-α) (30% in patients with T2DM) (Figure 2(a)) and IL-8 (20% in controls) (Figure 2(b)) production, and neutrophil IL-6 production was lower (60% in controls and 50% in patients with T2DM) in both groups following LPS stimulation (Figure 2(c))

Summary

Introduction

Neutrophil apoptosis, an important component of inflammation, regulates neutrophil functional longevity and resolution. Elevated levels of inflammatory markers [interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin (IL-8), and C-reactive protein (CRP)] were shown to increase neutrophil apoptosis and impair neutrophil clearance in patients with diabetes [2]. This dysfunction leads to the inadequate activation of leukocytes, advances the progression of diabetic complications, and increases the risk of invasive microorganisms [3,4,5]. We investigated the effects of a dance program on neutrophil number, function, and death in type 2 diabetes mellitus (T2DM) patients and healthy volunteers. Dance training is a nonpharmacological strategy to reduce inflammation and improve neutrophil clearance in patients with T2DM

Methods

Results

Discussion

Conclusion