Abstract
This chapter reviews a variety of culture methods and serologic tests that can be used in diagnoses of bovine mycoplasmas. Bovine semen contamination with mycoplasmas is a particular problem in both localized and geographic dissemination of infection through artificial insemination programs. Suitable diagnostic tests for such contamination are also outlined. The nutritional demands for isolation of the bovine mycoplasma species differ largely. Various media are needed to isolate bovine mycoplasmas, although most species can be isolated easily on modified Edward's media. Culture media make use of the different biochemical properties of the various mycoplasma species, in particular their ability to metabolize glucose, arginine, or urea. The media for Mycoplasma dispar and Ureaplasma diversum allow the growth of other, less fastidious mycoplasma species. Thus, when all possible mycoplasma species are isolated, various types of isolation media and their variants are used. The indirect immunofluorescence test is a convenient and reliable method to identify most mycoplasma species other than M. dispar.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
