Abstract
Background Amaranthus palmeri S. Watson is a serious problem in soybean crops from Argentina. This weed has evolved high level of resistance to (ALS)-inhibiting herbicides mainly due to a target-site mechanism by an altered ALS enzyme. In an A. palmeri population from Totoras with cross-resistance to (ALS)-inhibiting herbicides, six allelic versions of the ALS enzyme were identified. Objective The aim of this study was to evaluate plants from that resistant population carrying the ALS substitutions A122S, D376E or A205V, [...]
Highlights
Vegetative cloning procedure or by cross-pollination and dose-response it was confirmed that the A205V substitution conferred resistance to assays and herbicide single-dose tests were performed to evaluate in vivo herbicides in the triazolopyrimidines family
In vivo resistance levels to (ALS)-inhibiting herbicides were evaluated in vegetative clones carrying the D376E, A205V or A122S analysis of Totoras with cross-resistance to (ALS) substitutions (R1, R2 and R3 subpopulations, respectively)
Plants selected for cloning were screened from a resistant population of A. palmeri (Larran et al, 2017) by ALS sequencing (D376E, A205V) or by Derived cleaved amplified polymorphic sequence (dCAPS) (A122S) (Figure 2), according to each genotype frequency
Summary
Watson) is an extremely competitive weed given its huge stress tolerance, genetic variability, high growth rate, and reproduction capacity (Ward et al, 2013) It is a dioecious species, its reproductive biology favors the dispersion and recombination of alleles, speeding up herbicide resistance evolution along withother traits. Watson is a serious Results: Dose-response experiments showed that all the resistant problem in soybean crops from Argentina This weed has evolved high subpopulations survived at the highest doses tested (32 X) for imidazolinones, level of resistance to (ALS)-inhibiting herbicides mainly due to a target-site triazolopyrimidines and sulfonylureas, while the susceptible population was mechanism by an altered ALS enzyme. An analysis of Totoras with cross-resistance to (ALS)-inhibiting herbicides, six allelic the novel A122S substitution showed that it provides cross-resistance to five versions of the ALS enzyme were identified.
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