Abstract
Aim To evaluate the cytotoxicity, biocompatibility and mineralization capacity of BIO-C PULPO, and MTA.Methodology L929 fibroblasts were cultured and MTT assay was used to determine the material cytotoxicity on 6, 24, and 48 h. A total of 30 male rats (Wistar) aged between 4 and 6 months, weighing between 250 and 300 g were used. Polyethylene tubes containing BIO-C PULPO, MTA, and empty tubes were implanted into dorsal connective tissue. After the experimental periods (7, 15, 30, 60, and 90 days) the tubes were histologically analyzed using hematoxylin-eosin (H&E), immunolabeling of IL-1β and TNF-α, and von Kossa staining, or without staining for polarized light analysis. The average number of inflammatory cells was quantified; the mineralization assessment was determined by the area marked in μm2 and semiquantitative immunolabeling analyses of IL-1β and TNF-α were performed. Then, data underwent statistical analysis with a 5% significance level.Results It was observed that BIO-C PULPO and MTA presented cytocompatibility at 6, 24, and 48 similar or higher than control for all evaluated period. On periods 7 and 15 days, BIO-C PULPO was the material with the highest number of inflammatory cells (p<0.05). On periods 30, 60, and 90 days, BIO-C PULPO and MTA presented similar inflammatory reactions (p>0.05). No statistical differences were found between Control, BIO-C PULPO, and MTA for immunolabeling of IL-1β and TNF-α in the different periods of analysis (p<0.05). Positive von Kossa staining and birefringent structures under polarized light were observed in all analyzed periods in contact with both materials, but larger mineralization area was found with BIO-C PULPO on day 90 (p<0.05).ConclusionBIO-C PULPO was biocompatible and induced mineralization similar to MTA.
Highlights
Pulpotomy is the treatment for reversible pulpal lesions and it can be performed on permanent or deciduous teeth
Once the available literature lacks papers evaluating the biological properties of BIO-C PULPO, this study aimed to evaluate the cytotoxicity, inflammatory tissue response, mineralization capacity, and immunolabeling of interleukin (IL)-1β and tumor necrosis factor (TNA)-α of this material comparing gto white Mineral Trioxide Aggregate (MTA)-Ang (Angelus Industry, Londrina, Brazil)
At 6 hours, no statistically significant difference was found between white MTA-Ang and BIO-C PULPO (p>0.05)
Summary
Pulpotomy is the treatment for reversible pulpal lesions and it can be performed on permanent or deciduous teeth. After removal of the coronary pulp, a material must be chosen for insertion in the pulp region and this is a relevant factor that influences successful treatment.. After removal of the coronary pulp, a material must be chosen for insertion in the pulp region and this is a relevant factor that influences successful treatment.1-3 This material should be biocompatible, bactericidal, harmless to tissue, and promote healing of the pulp tissue.. The stimulus to mineralization is relevant, as it allows the deposit of a mineralized tissue barrier on the pulp surface.. The stimulus to mineralization is relevant, as it allows the deposit of a mineralized tissue barrier on the pulp surface.10 This mineral has appropriate radiopacity, low solubility, high pH, and antimicrobial activity. The stimulus to mineralization is relevant, as it allows the deposit of a mineralized tissue barrier on the pulp surface. this mineral has appropriate radiopacity, low solubility, high pH, and antimicrobial activity.
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