Cytotoxicity Effects of Azithromycin and Curative of Garlic on Sperm Mortality and Testicular Tissue of Albino Male Rats

Abstract

The current study aimed to identify the adverse effects of azithromycin drug and the protective role of garlic in cytotoxicity tests that included sperm deformation and histological changes in testes tissue of male albino rats. Twenty-four mature male rat animals (Rattus norvegicus) were used in the present study, their age was (2.5 – 3) months, and their weight ranged from 148 to 280 gm they were divided into four groups as follows:
 The first group was the control group contained four rats and was administered distilled water only. In contrast, the second group containing four rats was administered garlic at 480 mg/kg body weight. The third group included eight rats, was administered Azithromycin at the dose of 500 mg/kg body weight. The fourth group contained eight rats, and Azithromycin was administrated at 500 mg/k mg+ garlic at 480 mg/kg body weight. The groups were treated with Azithromycin and garlic daily. The results recorded a significant increase (p≥0.05) in sperm head and tail deformation rate in the group administered Azithromycin + garlic compared with the control groups that were administrated distilled water and garlic. Furthermore, the results reported a significant decrease (p≥0.05) in sperm head and tail deformation rates in the group administered Azithromycin + garlic compared with the only Azithromycin administered group. Moreover, the study indicated histological changes in testes tissue included degenerative changes represented by depletion of the spermatogonial cells (some seminiferous tubules appeared with one or two layers of spermatogonia) in the group that was administered Azithromycin compared with the control group, which was administered distilled water and garlic. In addition, the study indicated slight histological changes in testes tissue compared with standard testes architecture, somniferous tubules with the maturation of spermatogony, and sperms inside the lumen in groups administered Azithromycin and garlic compared with control groups, which were administered distilled water and garlic Finally, the findings showed a reduction in histological changes in testes tissue in the group that was administered azithromycin+garlic compared with the group that was administered Azithromycin only..