Abstract
Acetylspermine is polyamine analogue drug. It causes the generation of free radicals and induction of oxidative stress, associated with cellular injury. Proliferation cells assay with MTT of untreated MCF-7 cells was successively survived with maximum increment in its number after 48 h, whereas the survival cells of MCF-7 showed regular inhibition as it grown with different concentrations of acetylspermine. The maximum inhibitory effect of acetylspermine was shown at the dose 5.0 μM. The percent of inhibition reached 88% as compared with the corresponding control. The effect of acetylspermine on the antioxidant activities of MCF-7 demonstrated that the activity of both superoxide dismutase and peroxidase were increased as the concentration of acetylspermine increased up to 5.0 μM. The rate of increases was 5 and 3 folds more than their corresponding controls, whereas there was an obvious decline in the activities of polyphenol oxidase and catalase reaching the lowest values at the concentration 5.0 μM acetylspermine. Such previous controversial in the activities of the different enzymes manifested that acetylspermine application elevated the enzymes that induced the Reactive Oxygen Species (ROS). Moreover, our results proved that acetylspermine derivative treatment of MCF-7 causes morphological changes typical for apoptosis and plays an important role in destruction of cancer cells and the rate of destruction is dose dependent. The results clearly demonstrate that acetylspermine treatment augments the antioxidants defense mechanism, induced toxicity and provides evidence that it may have a therapeutic role in free radical mediated diseases.
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