Cytotoxic Isoprenylated Epoxycyclohexanediols from the Plant Endophyte Pestalotiopsis fici

Abstract

Aim To search for new cytotoxic secondary metabolites from solid cultures of the plant endophytic fungus Pestalotiopsis fici. Method Pestalofones F–H ( 1– 3) and pestalodiols A–D ( 4– 7) were isolated from the crude extract using silica gel, Sephadex LH-20 column chromatography, and reversed-phase HPLC. Their structures were elucidated by NMR spectroscopy. The new metabolites were tested for cytotoxicity against the HeLa and MCF-7 cells using the MTT method. Results Compounds 1– 3 and 6 showed cytotoxicity against HeLa and MCF-7 cells. Conclusion Seven new cytotoxic isoprenylated epoxycyclohexanediol derivatives were identified from the plant endophytic fungus Pestalotiopsis fici.