Cytotoxic Effects of Substance a Obtained from Oligosaccharides on Human Lung Cancer Cell Line, A549

Abstract

It is reported that oligosaccharides have antitumoric effects on cancer cells. To evaluate the in vivo antitumor potentials of oligosaccharides, we obtained substance A from them. In this study, we treated three kinds of cell lines(Hep3B, A549, 293T) with substance A in concentration of 2%, 1%, 0.5% respectively and incubated them 24 hours. Then we used MTT assay to measure viability of cells. As a result, the viability of A549 cells decreased as the concentration of substance A became higher while other cells(Hep3B, 293T) were almost same. Survival rates of A549 cells were 84%, 82% and 68% when treated with substance A in concentration of 0.5%, 1% and 2% respectively. In all concentrations, cytotoxic effects of substance A on A549 cells were about 5 times stronger than on 293T cells. Our results suggested that substance A has selective cytotoxic effects on A549 cell lines. This study demonstrated that substance A has antitumor effects on human lung cancer.