Cytotoxic Effect of Clethodim Exposure on Liver Cells&

Abstract

Clethodim, a herbicide group pesticide, is classified as a toxicity class II agent. The development toxicity, immunotoxicity, neurotoxicity and reproductive toxicity of this herbicide have been demonstrated in zebrafish and rat. There are no in vitro studies on the hepatoxic effects of clethodim. In this study, for the first time, clethodim was tested with liver cell line models. THLE-2 and B129 cells were treated with a wide range of clethodim for 96 hours. After that, its hepatoxic effects were evaluated by MTT and SRB analyses. Both assays revealed that the cellular proliferation was strikingly decreased with the dose dependent manner. Especially, 1000 µg/ml dose of the clethodim exposure supressed the cellular proliferation at a %90 and %75 for B129 and THLE-2 cells. Moreover, a significant reduction was acquired in both cells number and volume with the high doses of this cyclohexanedione class herbicide. These cytotoxic impacts can be strongly related to the herbicide elaved oxidative stress. In the light of our results, the long and high doses of clethodim exposure has a hepatoxic effect and its toxic target organ is be liver. Hence, further studies on the use of clethodim-based herbicides are urgently needed.