Abstract

Purpose: To evaluate the cytotoxicity of n-hexane extract and its metabolites obtained from the red alga, Laurencia majuscula, against three cancer cell lines HCT-116 (colon cancer), PC-3 (prostate cancer) and HepG2 (liver cancer) cells; and to identify the phytochemical compound(s) involved.
 Methods: Solvent extraction, thin layer chromatography, aluminum oxide column chromatography, and preparative thin layer chromatography (PTLC) were employed for isolating pure compounds from nhexane extract of Laurencia majuscula. Nuclear magnetic resonance (NMR) and mass spectrometry (MS) measurements were used for structural elucidation of the compounds. The cytotoxicity of the nonpolar extract and isolated compounds were evaluated against HCT, PC-3, and HepG2 cells using MTT assay, relative to the standard cytotoxic drug (cisplatin).
 Results: Three sesquiterpenes (1, 2 and 8), and five acetogenins (3-7) were isolated from the n-hexane extract. The n-hexane extract showed higher potent cytotoxic effect than sesquiterpenes and the acetogenins (3-7).
 Conclusion: These results indicate that the n-hexane extract of Laurencia majuscula exerts significant cytotoxicity against HCT-116, PC-3 and HepG2 cell lines, thus suggesting that the plant extract may be effective chemotherapeutic agents for the management of colon, postrate and liver cancer.
 Keywords: Red Sea alga, Rhodomelaceae, Polyketides, Terpenes, Anticancer

Highlights

  • Marine red algae comprise of diverse bioactive compounds that exert antimicrobial, antiinflammatory, cytotoxic, antifoulants, insecticidal and immunosuppressive effects [1,2]

  • It has been reported that the genus Laurencia is the most productive in the Rhodomelaceae genera

  • The present study was carried out to investigate the cytotoxicity of n-hexane extract of Laurencia majuscula, and to isolate and identify the Laurencia majuscula Lamouroux was collected in September, 2018 from Al-rays area, Saudi Arabia (23° 34` 11.3`` N; 38° 36` 10.6`` E)

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Summary

INTRODUCTION

Marine red algae comprise of diverse bioactive compounds that exert antimicrobial, antiinflammatory, cytotoxic, antifoulants, insecticidal and immunosuppressive effects [1,2]. Not much is known about the bioactive constituents of Laurencia majuscula responsible for its activity. The present study was carried out to investigate the cytotoxicity of n-hexane extract of Laurencia majuscula, and to isolate and identify the. Laurencia majuscula Lamouroux was collected in September, 2018 from Al-rays area, Saudi Arabia (23° 34` 11.3`` N; 38° 36` 10.6`` E). The three cell lines HCT-116 (colon cancer), PC3 (prostate cancer), and HepG2 (liver cancer) were purchased from American Type Culture Collection. 3.125, and 1.56 μg/mL) and after 48 h, the viability of each cancer cell line was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, 5 mg/mL) assay which measures the activity of mitochondrial succinate dehydrogenase in viable cells. All statistical analyses were performed using GraphPad InStat software, version 3.05 (GraphPad Software, La Jolla, CA). Graphs were plotted using GraphPad Prism software, version 6.00 (GraphPad Software, La Jolla, CA)

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