Cytotoxic and Genotoxic Effects of Methanol Extracts of Some Gypsophila L. Species

Ferhan Korkmaz,Ebru Ataşlar,Özgün Tuna Gülören,Meltem Erdir

doi:10.36253/caryologia-1091

Abstract

In this study, the cytotoxic and genotoxic effects of Gypsophila perfoliata L. var. perfoliata, Gypsophila perfoliata L. var. araratica Kit Tan, Gypsophila pilosa Hudson and Gypsophila osmangaziensis Ataşlar & Ocak plant extracts have been examined by using Allium Test method. Methanol extracts of plants have been prepared in 4 different concentrations (0.625 mg/ml, 1.250 mg/ml, 2.500 mg/ml and 5.000 mg/ml). After the onion roots were treated at these concentrations of plant extracts for 24 hours and 48 hours, mitosis preparations were prepared from these root tips. With the data being obtained by evaluating the preparations, mitotic index (%) and chromosome aberration (%) values have been calculated. Distilled water has been used as control group. It was found out that mitotic index and chromosome aberration values of all species showed significant differences compared to control group in the extract concentration range of 1.250–5.000 mg/ml. It has been determined that the most widely observed chromosome aberrations were disturbed metaphase, sticky metaphase, c-metaphase, disturbed anaphase and anaphase bridge.

Highlights

Gypsophila L., is a genus of Caryophyllaceae family, which is found between 30° and 60° latitudes and is represented with nearly 150 species in this region (Antkowiak and Dyba 2004, Schweingruber 2007)
(Chromosome Aberration %) of the methanol extracts of Gypsophila species used in the study are given in Table
In the 24-hour treatment, 5.000 mg / ml concentrationss significantly decreased Mitotic Index (MI)% in G. osmangiensis and G. pilosa extracts compared to the control group

Summary

Introduction

Gypsophila L., is a genus of Caryophyllaceae family, which is found between 30° and 60° latitudes and is represented with nearly 150 species in this region (Antkowiak and Dyba 2004, Schweingruber 2007). The cytotoxic and genotoxic effects of methanol extracts from four Gypsophila species, two of which are endemic, were investigated using Allium cepa root tip meristem cells. Cytotoxic and genotoxic effects of methanol extracts of vegetative parts of some Gypsophila species using Allium cepa assay consecutively three times. Genotoxicities of plant extracts used in the study have been determined with Allium Test.

Results

Conclusion