Abstract
Arsenic and lead, known to have genotoxic and mutagenic effects, are ubiquitously distributed in the environment. The presence of arsenic in drinking water has been a serious health problem in many countries. Human exposure to these metals has also increased due to rapid industrialization and their use in formulation of many products. Liposuction material is a rich source of stem cells. In the present study cytotoxic and genotoxic effects of these metals were tested on adipose derived mesenchymal stem cells (AMSCs). Cells were exposed to 1-10 μg/ml and 10-100 μg/ml concentration of arsenic and lead, respectively, for 6, 12, 24 and 48 h. The cytotoxic effects were measured by neutral red uptake assay, while the genotoxic effects were tested by comet assay. The growth of cells decreased with increasing concentration and the duration of exposure to arsenic. Even the morphology of cells was changed; they became round at 10 μg /ml of arsenic. The cell growth was also decreased after exposure to lead, though it proved to be less toxic when cells were exposed for longer duration. The cell morphology remained unchanged. DNA damage was observed in the metal treated cells. Different parameters of comet assay were investigated for control and treated cells which indicated more DNA damage in arsenic treated cells compared to that of lead. Intact nuclei were observed in control cells. Present study clearly demonstrates that both arsenic and lead have cytotoxic and genotoxic effects on AMSCs, though arsenic compared to lead has more deleterious effects on AMSCs.
Highlights
Stem cells are characterized by the ability to renew themselves and to differentiate into multi-lineage of terminally differentiated cells
Variable number of stem cells is present in different tissues such as liver, heart, kidney, skin, bone marrow and adipose tissue etc. [2], The tissues are mainly formed from mature cells but it is evident that stem cells reside in tissues in specific niches [3]
adipose derived mesenchymal stem cells (AMSCs) were successfully isolated from the single donor
Summary
Cytotoxic and Genotoxic effects of Arsenic and Lead on Human Adipose Derived Mesenchymal Stem Cells (AMSCs). In the present study cytotoxic and genotoxic effects of these metals were tested on adipose derived mesenchymal stem cells (AMSCs). Nearly 400,000 liposuction surgeries nearly 400,000 liposuction surgeries are performed in the USA per year, which yield approximately 100 ml to 3000 ml of lipoaspirate tissue It has great research and therapeutic applications, majority of this rich source of stem cells is wasted [9, 10]. We have investigated the cytotoxic as well as genotoxic effects of both these heavy metals on human adipose derived mesenchymal stem cells (AMSCs). Since there is no such study available on the effects of arsenic and lead on AMSCs, it becomes extremely relevant to explore the deleterious effects of both metals on stem cells
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
