Abstract
The combination of ascorbic acid and rutin, commonly used in oral preparations for their antioxidant and anti-inflammatory properties, can also be used to protect skin cells from the effects of UV radiation in sunlight. Here, we tested the potential protective effect of ascorbic acid and rutin used together in UVB-irradiated human skin fibroblasts, and assessed the proteomic profile of these cells, grown in a three-dimensional (3D) system. Proteomic findings revealed a combined effect of ascorbic acid and rutin in UV-irradiated fibroblasts against overexpression of pro-inflammatory signaling proteins and DNA reorganization/expression. These effects were not observed when cells were treated with either compounds alone. The antioxidant effects of ascorbic acid and rutin also prevented protein modifications by lipid peroxidation products. Further, ascorbic acid stimulated rutin-protein adduct formation, which supports intra/extracellular signaling and the Nrf2/ARE antioxidant pathway, contributing to the protective effects against UV-induced oxidative stress. The combined effect of ascorbic acid and rutin suggests that this combination of compounds is potentially effective against skin damage caused by UV radiation.
Highlights
UV radiation contained in sunlight is one of the common harmful environmental factors to which our skin is exposed daily
Irradiation onstudy, skin the cellscooperation can be reduced applicationantioxidants, of effective ascorbic acid supplied with food and rutin that is often used in pharmacy, were assessed for their role cytoprotective compounds
Results of this ascorbic acid supplied with foodinand rutin that is often used pharmacy,The were assessed forstudy their indicate significant changes the proteomic profile of UVB-irradiated treated with ascorbic role in the protection of theinproteome in UVB-irradiated human skinfibroblasts fibroblasts
Summary
UV radiation contained in sunlight is one of the common harmful environmental factors to which our skin is exposed daily As a result, this leads to the disruption of many metabolic pathways in cells responsible for building skin layers, which has been widely documented in the literature [1,2]. UV radiation, which mostly reaches the skin, contains high-energy UVB (280–320 nm) and highly penetrating UVA (320–400 nm) Both types of radiations lead to different biological effects; they have a common feature of intensifying the generation of the reactive oxygen species (ROS) [3]. Oxidative modifications of proteins affect their conformation, causing changes in their activity, which is important in the case of proteins with antioxidant and DNA repair properties and in signal molecules that are involved in the pro-inflammatory and proapoptotic response of skin cells [7,8]
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