Abstract
The radial segmentation (RS) of nuclei of human lymphocytes and monocytes was inhibited by vinblastine. Sulfhydryl reagents did not inhibit the formation of RS nuclei. The yield of RS nuclei after incubation in heparinized blood is probably due to the temperature during incubation and not to heparin, as evidenced by comparison with blood samples held fluid by defibrination with streptokinase. In the concentration range that potentiates muscle cell contraction caffeine had no significant influence on the formation of RS nuclei. These findings are discussed in relation to the hypothesis that microtubule shrinkage due to their slow decomposition is instrumental in the formation of RS nuclei.
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