Cytomorphological Indicators of Lymphoid Tissue and Protein Composition of Cattle Blood during the Prenatal and Postnatal Periods

Abstract

The cytomorphological reactions of lymphoid tissue and the immunobiological status of the cattle organism in the prenatal and postnatal periods were analyzed. The cytomorphological analysis was carried out by counting the lymphoid and plasma cells in 50 fields of vision according to the method in [10]. The level of metabolic processes in the experimental animals was determined by the content of the total protein in their blood serum estimated by refractometry [11]. Protein fractions were described with the electrophoresis in agar gel according to [12]. The immunoelectrophoresis was performed as described in [13]. The main shifts in the cellular composition of the lymph nodes and spleen, as well as other lymphoid tissues in early fetuses, were expressed in the accumulation of lymphoblasts, which were large basophilic cells. Electrophoretic separation and immune-based visualization of experimental animals' serum proteins were carried out simultaneously, and the phenomenon of complete antigenic identity was observed with both immune sera against cows’ and bulls’ blood proteins. From 18 to 22 precipitates were detected on their immunophoregrams.