Abstract
Reticulo-endothelial cells of liver, spleen, bone marrow, lymph nodes, thymus, subcutaneous connective tissue, omentum, mesentery, adrenal and hypophysis were demonstrated and defined by the classical vital staining technique with lithion carmine. Further, the increase of vitally stained cells and that of vital staining in a single cell by repeated injection of the dye-stuff or carbon particles were demonstrated and discussed, and it was shown clearly by the radio-auto-graphic technique with tritiated thymidine that Kupffer's stellate cells possessed more active mitotic activity than that of the liver cells. Cytochemical stainings adopted for demonstration of functional states of the reticulo-endothelial cells in fresh section cut with a criostat were PAS, Sudan black B, succinic dehydrogenase, labile and stable oxidase, peroxidase, phosphorylase, acid and alkaline phosphatase, esterase and aminopeptidase stain-ings, and it was concluded that esterase and acid phosphatase were mainly localized in the reticulo-endothelial cells and represented their functional states well.
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