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Abstract
BackgroundBoth photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Systematical investigating the regulatory mechanism of chloroplast development and chlorophyll (Chl) content variation is necessary for clarifying the photosynthesis mechanism for crops.ObjectiveThis study aims to explore the critical regulatory mechanism of leaf color mutation in a yellow–green leaf sesame mutant Siyl-1.MethodsWe performed the genetic analysis of the yellow-green leaf color mutation using the F2 population of the mutant Siyl-1. We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F2 progeny. We performed the two-dimensional gel electrophoresis (2-DE) and compared the protein expression variation between the mutant progeny and the wild type.ResultsGenetic analysis indicated that there were 3 phenotypes of the F2 population of the mutant Siyl-1, i.e., YY type with light-yellow leaf color (lethal); Yy type with yellow-green leaf color, and yy type with normal green leaf color. The yellow-green mutation was controlled by an incompletely dominant nuclear gene, Siyl-1. Compared with the wild genotype, the chloroplast number and the morphological structure in YY and Yy mutant lines varied evidently. The chlorophyll content also significantly decreased (P < 0.05). The 2-DE comparison showed that there were 98 differentially expressed proteins (DEPs) among YY, Yy, and yy lines. All the 98 DEPs were classified into 5 functional groups. Of which 82.7% DEPs proteins belonged to the photosynthesis and energy metabolism group.ConclusionThe results revealed the genetic character of yellow-green leaf color mutant Siyl-1. 98 DEPs were found in YY and Yy mutant compared with the wild genotype. The regulation pathway related with the yellow leaf trait mutation in sesame was analyzed for the first time. The findings supplied the basic theoretical and gene basis for leaf color and chloroplast development mechanism in sesame.
Highlights
Photosynthesis is widely considered as the basis of growth, development and yield in plants
98 differentially expressed proteins (DEPs) were found in YY and yellow-green leaf genotype (Yy) mutant compared with the wild genotype
The gene sequences for these proteins were obtained from the genome database of Yuzhi 11 and quantitative real-time PCR analysis
Summary
Photosynthesis is widely considered as the basis of growth, development and yield in plants. Many reports have shown that most leaf color related mutagenesis is involved in the structure and function variation of the chloroplasts (Li et al 2018a, b; Zhang et al 2006a, b), chlorophyll biosynthesis and degradation mechanisms (Oda-Yamamizo et al 2016), photosynthesis (Slattery et al 2017), and chloroplast development (Sugliani et al 2016; Leister 2003; Sang et al 2010). Most mutants currently belong to the second category (Zhang et al 2006a, b) Both photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Methods We performed the genetic analysis of the yellow-green leaf color mutation using the F2 population of the mutant Siyl-1. We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F2 progeny. The findings supplied the basic theoretical and gene basis for leaf color and chloroplast development mechanism in sesame
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