Abstract
The study was conducted to develop a cytology based diagnostic tool for the diagnosis of avian Mycoplasma and E. coli infections at post mortem in the field condition. A total of 38 culture and PCR confirmed Mycoplasma, E. coli or mixed infected samples were used for this study. Lung impression smears were prepared on glass slide from the samples at post mortem examination. Inflammatory cells were counted on microscope after Giemsa staining. Cell counts were analyzed with Bonferroni joint confidence interval and Mann-Whitney U test. The average cell percentages in healthy cases were 73.54- 81.66%, 9.63-13.37% and 7.42-14.38% for lymphocyte, heterophil, and macrophage, respectively. In case of Mycoplasma infection, average percentages of lymphocyte, heterophil and macrophages were 82.01-88.10%, 5.6 to 8.16% and 4.52 9.68%, respectively. In E. coli infection, average percentage of lymphocyte, heterophil and macrophages were found as 64.44-70.76%, 19.73-23.47% and 8.9- 12.7%, respectively. In mixed infection, lymphocyte, heterophil and macrophage were found as 76.08-80.50%, 13.47 17.63% and 4.56 7.66%, respectively. Statistical analyses revealed that in Mycoplasma infection number of lymphocyte and in E. coli infection number of heterophil increased significantly (p< 0.01). In MC complex, number of heterophil increased and macrophages decreased significantly (p < 0.01). These findings could help identification of Mycoplasma, E. coli or Mycoplasma- E. coli complex at post mortem examination in the field condition. DOI: http://dx.doi.org/10.3329/sja.v10i2.18331 SAARC J. Agri., 10(2): 95-105 (2012)
Highlights
Poultry rearing is playing a vital role in the poverty alleviation and income generation in Bangladesh
This paper describes the use of cytological smear collected at necropsy for the diagnosis of avian Mycoplasma and E. coli infections which were compared with the histopathological changes
The samples that were confirmed as Mycoplasma or E. coli or mixed infection by culture and Polymerase chain reaction (PCR) were subjected for cytological evaluation
Summary
Poultry rearing is playing a vital role in the poverty alleviation and income generation in Bangladesh. Mycoplasmosis is an economically important disease of poultry caused by four commonly recognized pathogens – Mycoplasma gallisepticum, M. synoviae, M. meleagridis and M. iowae. In Bangladesh, the prevalence of Mycoplasmosis markedly increases in the winter season and may reach up to 61.45% which is a threat for poultry sector (Sikder et al, 2005). Very often it is confused with other respiratory diseases with E. coli infection which sometimes make the treatment very expensive. Clinical pathology has played a major role in the diagnosis of human and animal diseases. This paper describes the use of cytological smear collected at necropsy for the diagnosis of avian Mycoplasma and E. coli infections which were compared with the histopathological changes
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