Abstract
The role of PLD in the molecular mechanism of cytokinin action remains to be elucidated. Data on the effect of cytokinins on PLD activity are practically missing. In view of this, the goal of this work was to study the possible role of PLD in cytokinin signal transduction. The objectives of this study were: to test the compounds that disturb the function of PLD in the test for cytokinins and to determine the character of effect of cytokinins on PLD in vivo at early stages of hormone action. The main result of this work was the discovery of a rapid activation of PLD in vivo in the presence of cytokinins. This finding, along with the data on the suppression of cytokinin effect by PLD inhibitors, testifies to the involvement of phospholi� pase D in the intracellular cytokinin signal transduc� tion. The effect of cytokinins on sensitive plant tissues and PLD activity was studied in biological tests with cotyledons of the amaranth ( Amaranthus caudatus L.) etiolated seedlings. Amaranth cotyledons are highly sensitive to exogenous cytokinins and respond to the presence of cytokinins in the dark by a rapid accumu� lation of the red pigment amaranthin. This reaction
Published Version
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