Cytokine-inducible growth factor gene expression in human umbilical endothelial cells

Abstract

Endothelial cells are known to be involved in different growth promoting processes like angioneogenesis, atherosclerosis or haematopoiesis. A great number of polypeptide growth factors crucial in this context have been isolated and they may be expressed in endothelial cells in either a constitutional or an inducible manner. The aim of the study was to examine the cytokine-inducibility of growth factor gene expression in endothelial cells. As uniform stimulators interleukin 1-α (IL-1α) and tumour necrosis factor (TNF)-α were chosen. Human umbilical arterial endothelial cells (HUAEC) were treated with either IL-1α or TNF-α and the gene expression of various growth factors was detected by reverse transcription-polymerase chain reaction (RT-PCR). We could demonstrate in HUAEC that stimulation with IL-1α- and TNF-α led to the mRNA expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) which are crucial in the process of angioneogenesis and atherosclerosis as well as of the granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF) and stem cell factor (SCF) which are main growth factors in haematopoiesis. The demonstration of the inducibility of a wide range of various growth factor genes in endothelial cells is of major interest regarding the growth regulatory role of the endothelium.