Cytogenotoxic effect of trichothecene T2 toxin on Allium sativum root tip meristematic cells

Abstract

Trichothecene T2 is a mycotoxin from the Fusarium species. This research aims to test the effect of the Trichothecene T2 toxin on mitotic index% (M.I.%) and induction of mitotic aberrations by using the Allium sativum (garlic) test system. The toxin concentrations in ppm were: 0.00, 0.30, 0.60, 0.90, and 1.20 for 12 hours. The garlic roots were then cut and mitotic slides were prepared by the squash method and examined under a light microscope. The results revealed that the mycotoxin has a significant mitodepressive effect at all concentrations compared to the control and the MI% reduction was proportional to increasing toxin concentration. The highest reduction in mitotic index was observed in the 1.2 ppm treatment. Moreover, this mycotoxin induced and increased the rate of mitotic abnormalities% (MA%) with increasing the mycotoxin concentration. The observed mitotic abnormalities were star-shaped anaphase, sticky metaphase, C-mitosis, sticky anaphase, depolarization, micronuclei, laggard chromosomes, anaphase bridges, and chromosome loss. The least frequently observed abnormality was micronuclei compared to the most frequent aberration, laggard chromosomes. The total mitotic abnormalities significantly increased with increasing the toxin dose concentration. These results suggest that this mycotoxin can inhibit the mitotic activity of the meristematic cells, it is mutagenic and can disrupt the spindle fibers activity of the dividing cells at all concentrations, especially at its higher doses in food. Therefore, the foods must be tested for fungi producing this mycotoxin. Keywords: Mycotoxin; mitodepressive; root tip; spindle fibers; mutagenic