Cytogenomic characteristics of murine breast cancer cell line JC

Shaymaa Azawi,Martina Rincic,Thomas Liehr

doi:10.1186/s13039-020-00524-z

Shaymaa Azawi, Martina Rincic + Show 1 more

Open Access

https://doi.org/10.1186/s13039-020-00524-z

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Abstract

BackgroundBreast cancer (BC), one of the most frequent human tumors, is genetically and histologically heterogeneous. Treatment options can be adapted according to BC subtype. Still, research is necessary to characterize BC biology better and to study potential new treatment options. Murine BC-cell lines can be used as model systems in this respect.ResultsHere for the first time murine BC-cell line JC was cytogenomically characterized as being complex rearranged and near-tetraploid. Multicolor banding and array comparative genomic hybridization were applied and the result was in silico translated to the human genome.ConclusionsEven though being commercially available, cell line JC was yet not much included in BC-research, most likely due to a lack of cytogenomic data. Thus, here comprehensive data is provided on chromosomal aberrations, genomic imbalances and involved breakpoints of JC cell line. Also JC could be characterized as a model for BC of luminal B type, basal-like tumor rather than for luminal A type.

Highlights

Breast cancer (BC) is considered to be one of the most aggressive human cancer forms and is leading among cancer-related deaths, especially in females
BCs are a heterogenic group of tumors, and divided into subtypes, according to their molecular profiles, morphology, and expression of specific biomarkers [4]
Cell lines The cell line JC was obtained from American Type Culture Collection (ATCCR CRL-2116TM, Wesel, Germany)

Summary

Introduction

Breast cancer (BC) is considered to be one of the most aggressive human cancer forms and is leading among cancer-related deaths, especially in females. Immunohistochemical markers for tumor cell surface or plasma, as well as for growth in this context are estrogen receptor, progesterone receptor, human epidermal growth factor receptor-2 (ERBB2/ HER-2) and epidermal growth factor receptor, cytokeratin 5 and/or nuclear protein Ki67 expression [1, 7]. Based on such expression profiles BC can be classified in (1) luminal A-like, (2) luminal B-like (HER2-positive or HER2-negative), (3) HER2-overexpressing, and (4) triplenegative subtypes [4, 7]. Murine BC-cell lines can be used as model systems in this respect

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