Abstract
Aim is to assess repeatedly cytogenetic effects of co- or monoinfection caused by Lyme borreliosis and tick-borne encephalitis during the acute and convalescent periods of the disease depending on variants of glutathione-S-transferase (GSTM1 or GSTT1) genes in the patient’s genotype. Material and methods: The study included 186 patients and 166 healthy (control) residents of the north of the Tomsk and Tyumen regions, who were examined by clinical, laboratory and cytogenetic methods (micronucleus analysis). Among the 186 examined patients, Lyme borreliosis was diagnosed in 65 individuals, tick-borne encephalitis was in 59 patients, and coinfection was found in 62 individuals. The material for the study (smears of buccal cells) was obtained repeatedly during admission of patients to treatment, and also after 1 week, 1, 3 and 6 months. Polymerase chain reaction was used to analyze the alleles of the GSTM1 and GSTT1 genes. Results: significant increase in the frequency of micronucleated buccal cells in patients with coinfection, as compared with the groups of control and patients with monoinfection. The significantly increased frequency of micronucleated cells was associated with the mutant inactive alleles of the GSTM1(0/0) and GSTT1(0/0) genes. If the patients were carriers of the mutant allele of the GSTM1(0/0) gene, the cytogenetic instability could persist for half a year. It was found that chronic arthritis in the Lyme borreliosis patients was associated with a long persistence of an increased frequency of micronucleated cells. Conclusion: Significant differences in the frequency and the lasting of persistence of micronucleated cells between groups of patients with coinfection and monoinfections were found. The most significant increase in those parameters was detected in the coinfected patients in whose genotype contained non-active forms of the GSTM1(0/0) and GSTT1(0/0) genes.
Highlights
repeatedly cytogenetic effects of co- or monoinfection caused by Lyme borreliosis and tick-borne encephalitis
convalescent periods of the disease depending on variants of glutathione-S-transferase
who were examined by clinical, laboratory
Summary
ЦИТОГЕНЕТИЧЕСКИЕ ПОСЛЕДСТВИЯ МИКСТИЛИ МОНОКЛЕЩЕВЫХ ИНФЕКЦИЙ В ЗАВИСИМОСТИ ОТ ВАРИАНТОВ ГЕНОВ ГЛУТАТИОН-S-ТРАНСФЕРАЗЫ (GSTM1 или GSTT1) В ГЕНОТИПЕ БОЛЬНОГО. Резюме Цель: провести оценку в динамике цитогенетичес ких последствий микст- и моноинфекций, вызванных иксодовым клещевым боррелиозом и клещевым энцефалитом, в острый и реконвалесцентный периоды болезни в зависимости от вариантов генов глутатион-Sтрансферазы (GSTM1 или GSTT1) в генотипе больного. Материал для исследования (мазки клеток буккального эпителия) был получен в динамике при госпитализации, а также через 1 неделю, 1, 3 и 6 месяцев. Результаты: существенное повышение частоты клеток буккального эпителия с микроядрами у больных с микcт-инфекцией, по сравнению с группами больных с моноинфекциями и контролем. Наиболее существенно повышенная частота клеток с микроядрами была связана с мутантными неактивными аллелями генов GSTM1(0/0) и GSTT1(0/0). Что хронический артрит у больных иксодовым клещевым боррелиозом сопровождался длительным персистированием повышенной частоты буккальных клеток с микроядрами. Заключение: установлены существенные различия в частоте и длительности персистирования клеток буккального эпителия с микроядрами между группами
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