Abstract
BackgroundThree-dimensional (3D) printing is widely used in the fabrication of dental prostheses; however, the influence of dental materials used for 3D printing on temporary restoration of fibroblasts in tissues is unclear. Thus, the influence of different dental materials on fibroblasts were investigated.MethodsDigital light processing (DLP) type 3D printing was used. Specimens in the control group were fabricated by mixing liquid and powder self-curing resin restoration materials. The temporary resin materials used were Model, Castable, Clear-SG, Tray, and Temporary, and the self-curing resin materials used were Lang dental, Alike, Milky blue, TOKVSO CUREFAST, and UniFast III. Fibroblast cells were cultured on each specimen and subsequently post-treated for analysis. Morphology of the adhered cells were observed using a confocal laser scanning microscope (CLSM) and a scanning electron microscope (SEM).ResultsCLSM and SEM cell imaging revealed that the 3D printed material group presented better cell adhesion with well-distributed filopodia compared to that in the conventional resin material group. Cell proliferation was significantly higher in the 3D printing materials.ConclusionSuperior cytocompatibility of the specimens fabricated through 3D printing and polishing process was demonstrated with the proof of better cell adhesion and higher cell proliferation.
Highlights
Three-dimensional (3D) printing is widely used in the fabrication of dental prostheses; the influence of dental materials used for 3D printing on temporary restoration of fibroblasts in tissues is unclear
Cell attachment analysis The morphology and adhesion of fibroblasts in this invitro cell experiment using the specimens fabricated by 3D printing and self-curing resin technology are presented in Figs. 1, 2
In accordance with the experiment finding, the null hypothesis was rejected, and this study revealed that there were statistically significant differences in fibroblast cytocompatibility between 3D printing technology-based and self-curing technologybased temporary restorative resin materials
Summary
Three-dimensional (3D) printing is widely used in the fabrication of dental prostheses; the influence of dental materials used for 3D printing on temporary restoration of fibroblasts in tissues is unclear. Temporary restoration materials are widely used in dental clinics and are important for predicting the successful prognosis of endodontic treatment including inlay, onlay, crown, and bridge [1, 2]. These materials are used to protect the invasion of external substances and microorganism and to help the recovery of tooth functions, including mastication and esthetics [3, 4]. The powder contains poly-methyl methacrylate (PMMA), a reaction initiator, and the liquid contains methyl methacrylate (MMA) and a small amount of inhibitor [8, 9]. The conditions of the work environment are strict and the process is, time consuming
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