Cytochrome P450 1B1 Overexpression in Cervical Cancers: Cross-sectional Study.

Fatemah O F O Alshammari,Yousef M Al-Saraireh,Ahmed M M Youssef,Hamzeh Mohammad Alrawashdeh,Yahya M Al-Sarayra

doi:10.2196/31150

Abstract

BackgroundCurrent standard treatments for patients with recurrent cervical cancer are not very effective and are associated with severe toxicity. Recently, the rational approach for the discovery of new therapies for cervical cancer is based on the alterations in the molecular biology of cancer cells. One of the emerging molecular changes in cancer cells is the aberrant expression of cytochrome P450 1B1 (CYP1B1). This unique enzyme has been reported to be selectively overexpressed in several cancers.ObjectiveThe aim of this study was to examine CYP1B1 expression in cervical cancers and to assess the enzyme’s relationship with several clinicopathological features.MethodsImmunohistochemistry was performed to examine CYP1B1 expression in 100 patient samples with cervical cancer and 10 patient samples with normal healthy cervical tissues.ResultsCYP1B1 was expressed in the majority of the cervical cancer samples (91/100, 91.0%) but not in normal healthy cervical samples. The difference in the expression of CYP1B1 between healthy and tumorous cervical tissues was significant (P=.01). Moreover, the frequency of CYP1B1 expression was found to be significantly higher in patients with advanced grades of the disease (P=.03) and in patients having metastasis to the lymph nodes (P=.01). Surprisingly, there was a significantly higher expression of CYP1B1 in patients with a high prevalence of human papilloma virus 16/18 (P=.04).ConclusionsThe differential profile of CYP1B1 expression between cervical cancer tissues and normal cervical tissues suggests that CYP1B1 may be used as a target for future therapeutic exploitations.

Highlights

Cervical cancer is the fourth most frequent cause of cancer deaths and morbidity in women around the world [1,2]
Given that no previous studies have examined the expression of cytochrome P450 1B1 (CYP1B1) in cervical cancers, this study aims to assess the aberrant expression of CYP1B1 in cervical cancer and its relation to baseline demographic and clinicopathologic features
Data on demographic and clinicopathologic features were available for 110 patient cases. These cases included 100 patients diagnosed with cervical cancer and 10 cases with normal cervix pathology presented as control

Summary

Introduction

Cervical cancer is the fourth most frequent cause of cancer deaths and morbidity in women around the world [1,2]. The primary cause of cervical cancer development is persistent infection with human papilloma virus (HPV), in particular, HPV 16 and HPV 18, this is not the only factor. These 2 aggressive types of viruses are responsible for causing about 70% of cervical cancers [4,5]. One of the emerging molecular changes in cancer cells is the aberrant expression of cytochrome P450 1B1 (CYP1B1). Methods: Immunohistochemistry was performed to examine CYP1B1 expression in 100 patient samples with cervical cancer and 10 patient samples with normal healthy cervical tissues. Conclusions: The differential profile of CYP1B1 expression between cervical cancer tissues and normal cervical tissues suggests that CYP1B1 may be used as a target for future therapeutic exploitations

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