Abstract
Cytochrome oxidase (EC.1.9.3.1) and NADH cytochromec reductase (EC.1.6.2.1) were analyzed during the process of skin wound healing in young adult and adult rats. Major spurts of cytochrome oxidase activity occurred between the 3rd–5th and 3rd–10th postoperative day respectively is adult, and young adult skin. The activity declined sharply between the 5th–7th day, and remained elevated between the 5th–10th day respectively in mature and young adult tissue. NADH cytochromec reductase inceased between the 3rd–10th postoperative day reaching a peak on the 10th day in both age groups. The enzymatic activity declined towards that of its normal level between the 10th and 15th day after injury. The overcompensatory response observed in the distribution of cytochrome oxidase was not observed in NADH cytochromec reductase, suggesting that the enzyme markers reflect two different processes occuring during regeneration.
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