Abstract
Cytochrome levels in control and concanavalin A-treated lymphocytes were quantitated using low-temperature spectroscopy (77 °K) and correlated with cell protein. Cytochrome ratios in uncultured lymphocytes of 1.00:0.28:1.86 were estimated from substrate-reduced spectra for cytochromes c, b, and aa 3, respectively. Control samples in culture showed a significant 20-h increase and 36-h decrease in cytochromes b and aa 3. These alterations are believed to be a consequence of isolation treatment and/or placement in a tissue culture environment. Concanavalin A-treated lymphocytes exhibited marked increases at 36 and 45 h in cytochrome b and dithionite-reduced cytochrome aa 3. These increases occur after the 20 h required for commitment to mitogenesis and appear to represent synchronous synthesis correlated to the G 2 phase of the cell cycle. Both control and concanavalin A-treated cells exhibited significant changes in cytochrome b 5. Mitochondrial involvement in mitogenesis is supported by evidence indicating that the mitogenic process as well as concanavalin A-induced cytochrome changes are inhibited by chloramphenicol. The changes observed in cytochrome b 5 are not significantly affected by chloramphenicol.
Published Version
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